The Anticancer Drug 3-Bromopyruvate Induces DNA Damage Potentially Through Reactive Oxygen Species in Yeast and in Human Cancer Cells - General Discussion, Q&A https://forum.worldhealth.net/community/discussion/the-anticancer-drug-3-bromopyruvate-induces-dna-damage-potentially-through-reactive-oxygen-species-in-yeast-and-in-human-cancer-cells/ World Health Network Discussion Board en-US Tue, 14 Jul 2026 12:56:22 +0000 wpForo 60 The Anticancer Drug 3-Bromopyruvate Induces DNA Damage Potentially Through Reactive Oxygen Species in Yeast and in Human Cancer Cells https://forum.worldhealth.net/community/discussion/the-anticancer-drug-3-bromopyruvate-induces-dna-damage-potentially-through-reactive-oxygen-species-in-yeast-and-in-human-cancer-cells/#post-1910 Mon, 15 Jan 2024 01:57:08 +0000 3-bromopyruvate (3-BP) is a small molecule with anticancer and antimicrobial activities. 3-BP is taken up selectively by cancer cells’ mono-carboxylate transporters (MCTs), which are highly overexpressed by many cancers. When 3-BP enters cancer cells it inactivates several glycolytic and mitochondrial enzymes, leading to ATP depletion and the generation of reactive oxygen species. While mechanisms of 3-BP uptake and its influence on cell metabolism are well understood, the impact of 3-BP at certain concentrations on DNA integrity has never been investigated in detail. Here we have collected several lines of evidence suggesting that 3-BP induces DNA damage probably as a result of ROS generation, in both yeast and human cancer cells, when its concentration is sufficiently low and most cells are still viable. We also demonstrate that in yeast 3-BP treatment leads to generation of DNA double-strand breaks only in S-phase of the cell cycle, possibly as a result of oxidative DNA damage. This leads to DNA damage, checkpoint activation and focal accumulation of the DNA response proteins. Interestingly, in human cancer cells exposure to 3-BP also induces DNA breaks that trigger H2A.X phosphorylation. Our current data shed new light on the mechanisms by which a sufficiently low concentration of 3-BP can induce cytotoxicity at the DNA level, a finding that might be important for the future design of anticancer therapies.

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The Anticancer Drug 3-Bromopyruvate Induces DNA Damage Potentially Through Reactive Oxygen Species in Yeast and in Human Cancer Cells - PMC (nih.gov)

]]> General Discussion, Q&A Bromopyruvate DNA https://forum.worldhealth.net/community/discussion/the-anticancer-drug-3-bromopyruvate-induces-dna-damage-potentially-through-reactive-oxygen-species-in-yeast-and-in-human-cancer-cells/#post-1910