An Introduction to Medical Ozone Therapy with Dr. Frank Shallenberger

Frank A. Shallenberger, MD, HMD, ABAARM, is the founder of the Nevada Center of Alternative & Anti-Aging Medicine and a longtime leader in clinical ozone therapy. A dual licensee in Nevada (MD and Homeopathic MD), he integrates conventional and homeopathic approaches in practice. Dr. Shallenberger is President Emeritus and program director of the American Academy of Ozonotherapy (AAOT) and an original appointee to ISCO3 when the international scientific committee was founded. He created Prolozone® injection therapy and developed Bio-Energy Testing® to assess oxygen utilization and mitochondrial function. He edits the Second Opinion medical newsletter.

Credentials at a Glance

• Medical training: MD, University of Maryland School of Medicine; postgraduate training at Mt. Zion Hospital, San Francisco. Former Clinical Instructor, UC Davis School of Medicine.

• Licensure: Nevada physician (MD) and Nevada Homeopathic MD (HMD).

• Leadership: President Emeritus, Board Member & Conference Program Director, AAOT. Appointed member at the founding of ISCO3 (International Scientific Committee of Ozone Therapy).

• Innovations: Originator of Prolozone® injection therapy; developer of Bio-Energy Testing® for clinical assessment of oxygen utilization and mitochondrial function.

• Intellectual property: U.S. Patent US7273453B2 on methods to analyze biological age using VO₂/RER-based respiratory gas metrics.

• Education & training impact: Has taught ozone therapy to physicians since the early 1990s; AAOT conference program lead; more than 700 clinicians trained.

• Editorial: Editor of the Second Opinion newsletter.

More on Dr. Frank

AAOT leadership: Dr. Shallenberger is President Emeritus, board member, and conference program director, underscoring his ongoing roles in standards, education, and meetings.

International standards work: He was among the inaugural appointees to ISCO3, contributing to global ozone therapy guidelines and technical documents.

Prolozone® origin and scope: In the Journal of Prolotherapy, he described Prolozone® as combining neural therapy and prolotherapy with oxygen-ozone gas to treat degenerative and painful conditions.

Oxygen-utilization focus: His Bio-Energy Testing® program measures VO₂/VCO₂ at rest and exertion to infer mitochondrial efficiency and fat metabolism, reflected in his U.S. patent methodology for calculating biological age.

Teaching history: He has trained physicians in ozone therapy since the early 1990s and continues to lead AAOT educational programs.

Dual-track licensure: Nevada’s official registry lists him as both an MD and Homeopathic MD.

Selected Works & Resources

• Books: The Principles and Applications of Ozone Therapy (2011); The Ozone Miracle (2017); Bursting With Energy; The Type 2 Diabetes Breakthrough.

• Peer-oriented article: Shallenberger F. ‘Prolozone™ – Regenerating Joints and Eliminating Pain.’ Journal of Prolotherapy, 2011.

• Patent: US7273453B2 ‘Method for analyzing the biological age of a subject’ (respiratory gas analysis/VO₂-RER metrics).

• Professional organizations: American Academy of Ozonotherapy (AAOT), International Scientific Committee of Ozone Therapy (ISCO3).

• Editorial: Editor of the Second Opinion newsletter.